Horse insulin-like growth factor 1 (IGF1) ELISA kit

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产品名称：Cusabio/Horse insulin-like growth factor 1 (IGF1) ELISA kit/96T,5×96T,10×96T/CSB-EL011086HO

产品编号：CSB-EL011086HO

市场价：¥0.00

场地：美国(厂家直采)

产品分类：仪器类>其它类仪器>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：待定

品牌： Cusabio

公司：Cusabio

公司分类：

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Horse insulin-like growth factor 1 (IGF1) ELISA kit

^Instructions

Code	CSB-EL011086HO
Size	96T,5×96T,10×96T
Trial Size
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Target Data

Target Name

insulin-like growth factor 1 (somatomedin C)

Alternative Names

IGF1 ELISA Kit; Insulin-like growth factor I ELISA Kit; IGF-I ELISA Kit; Somatomedin ELISA Kit; Fragment ELISA Kit

Abbreviation

IGF1

Uniprot No.

P51458

Species

Equus caballus (Horse)

Sample Types

serum, plasma, tissue homogenates

Detection Range

12.5 ng/mL-800 ng/mL

Sensitivity

3.12 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of horse IGF1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	89-95
1:2	Average %	95
	Range %	90-104
1:4	Average %	92
	Range %	87-100
1:8	Average %	89
	Range %	80-97

Recovery

The recovery of horse IGF1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	87-101
EDTA plasma (n=4)	87	82-93

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
800	1.961	1.944	1.953	1.820
400	1.488	1.605	1.547	1.414
200	1.178	1.213	1.196	1.063
100	0.912	0.902	0.907	0.774
50	0.669	0.657	0.663	0.530
25	0.405	0.396	0.401	0.268
12.5	0.260	0.249	0.255	0.122
0	0.137	0.129	0.133

Troubleshooting and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function	The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation. Ca(2+)-dependent exocytosis of IGF1 is required for sensory perception of smell in the olfactory bulb. Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiatiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins ITGAV
Subcellular Location	Secreted
Protein Families	Insulin family
Database Links	STRING: 9796.ENSECAP00000008158 UniGene: Eca.12985

品牌介绍

武汉华美生物工程有限公司成立于2007年12月，注册资金为5000万元，是一家集科研、生产、销售为一体的国家高新技术企业，以食品安全检测产品生产、诊断试剂原料供应、科研试剂生产、生物技术服务为核心，主要为国内外知名的医药研发公司、诊断试剂生产厂家、政府监管检测机构、高等院校、企业及科研院所等提供相关产品及定制化的技术服务。公司拥有6个研发平台，分别为食品安全检测产品开发平台、高通量基因克隆平台、蛋白质组学的生物标记物高通量筛选平台、生物标记物的免疫性检测方法快速建立平台、四大表达系统的重组蛋白表达平台和科研及诊断用单多抗制备平台。以技术为核心、视质量为生命、奉用户为上帝，竭诚为您提供性价比较高的检测产品及无微不至的售后服务。业务范围主营基因、重组蛋白、抗体、ELISA试剂盒、食品安全检测试剂盒及诊断试剂原料产品。

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