Target Name | cholecystokinin octapeptide,CCK-8 |
Abbreviation | CCK-8 |
Species | Rattus norvegicus (Rat) |
Sample Types | serum, plasma, tissue homogenates |
Detection Range | 31.25 pg/mL-2000 pg/mL |
Sensitivity | 7.8 pg/mL |
Assay Time | 1-5h |
Sample Volume | 50-100ul |
Detection Wavelength | 450 nm |
Research Area | Metabolism |
Assay Principle | quantitative |
Measurement | Sandwich |
Precision | Intra-assay Precision (Precision within an assay):CV%<8% | | | | | Threesamples of known concentration were tested twenty times on one plate toassess. | | | Inter-assay Precision (Precision between assays): CV%<10% | | | | | Threesamples of known concentration were tested in twenty assays to assess. | | | | | | | | | | | |
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Linearity | Toassess the linearity of the assay, samples were spiked with highconcentrations of rat CCK-8in various matrices and diluted with theSample Diluent to produce samples with values within the dynamic range of theassay. | | | Sample | Serum(n=4) | | | 1:1 | Average % | 98 | | | Range % | 94-103 | | | 1:2 | Average % | 85 | | | Range % | 80-90 | | | 1:4 | Average % | 92 | | | Range % | 88-93 | | | 1:8 | Average % | 89 | | | Range % | 84-92 | | |
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Recovery | Therecovery of rat CCK-8 spiked to levels throughout the range of the assay invarious matrices was evaluated. Samples were diluted prior to assay asdirected in the Sample Preparation section. | | | Sample Type | Average % Recovery | Range | | | Serum (n=5) | 94 | 90-97 | | | EDTA plasma (n=4) | 86 | 83-89 | | | | | | | | | | | | | | | | | | |
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Typical Data | Thesestandard curves are provided for demonstration only. A standard curve shouldbe generated for each set of samples assayed. | | |  pg/ml | OD1 | OD2 | Average | Corrected | | | 2000 | 1.889 | 2.000 | 1.945 | 1.843 | | | 1000 | 1.463 | 1.501 | 1.482 | 1.380 | | | 500 | 0.961 | 0.932 | 0.947 | 0.845 | | | 250 | 0.603 | 0.612 | 0.608 | 0.506 | | | 125 | 0.405 | 0.389 | 0.397 | 0.295 | | | 62.5 | 0.237 | 0.241 | 0.239 | 0.137 | | | 31.25 | 0.143 | 0.150 | 0.147 | 0.045 | | | 0 | 0.100 | 0.104 | 0.102 | | | |
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Troubleshooting and FAQs | ELISA kit FAQs |
Storage | Store at 2-8°C. Please refer to protocol. |
Lead Time | 7-14 working days |