Target Name | midregional pro-atrial natriuretic peptide (MR-proANP) |
Abbreviation | MR-proANP |
Species | Homo sapiens (Human) |
Sample Types | serum, plasma, tissue homogenates |
Detection Range | 39 pmol/L-2500 pmol/L |
Sensitivity | 9.8 pmol/L |
Assay Time | 1-5h |
Sample Volume | 50-100ul |
Detection Wavelength | 450 nm |
Research Area | Others |
Assay Principle | quantitative |
Measurement | Sandwich |
Precision | Intra-assay Precision (Precision within an assay): CV%<8% | | | | Three samples of known concentration were tested twenty times on one plate to assess. | | Inter-assay Precision (Precision between assays): CV%<10% | | | | Three samples of known concentration were tested in twenty assays to assess. | | | | | | | | | |
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Linearity | To assess the linearity of the assay, samples were spiked with high concentrations of human MR-proANP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | | Sample | Serum(n=4) | | 1:1 | Average % | 94 | | Range % | 85-97 | | 1:2 | Average % | 100 | | Range % | 94-105 | | 1:4 | Average % | 90 | | Range % | 83-94 | | 1:8 | Average % | 97 | | Range % | 91-101 | |
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Recovery | The recovery of human MR-proANP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
Sample Type | Average % Recovery | Range | | Serum (n=5) | 95 | 89-98 | | EDTA plasma (n=4) | 102 | 92-107 | | | | | | | | | | | | | | | |
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Typical Data | These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
 pmol/L | OD1 | OD2 | Average | Corrected | | 2500 | 2.155 | 1.988 | 2.072 | 1.926 | | 1250 | 1.697 | 1.627 | 1.662 | 1.516 | | 625 | 1.160 | 1.088 | 1.124 | 0.978 | | 312 | 0.692 | 0.708 | 0.700 | 0.554 | | 156 | 0.414 | 0.425 | 0.420 | 0.274 | | 78 | 0.283 | 0.277 | 0.280 | 0.134 | | 39 | 0.197 | 0.191 | 0.194 | 0.048 | | 0 | 0.148 | 0.144 | 0.146 | | |
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Troubleshooting and FAQs | ELISA kit FAQs |
Storage | Store at 2-8°C. Please refer to protocol. |
Lead Time | 3-5 working days |