| Target Name | midregional pro-atrial natriuretic peptide (MR-proANP) |
| Abbreviation | MR-proANP |
| Species | Homo sapiens (Human) |
| Sample Types | serum, plasma, tissue homogenates |
| Detection Range | 39 pmol/L-2500 pmol/L |
| Sensitivity | 9.8 pmol/L |
| Assay Time | 1-5h |
| Sample Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Others |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | | Intra-assay Precision (Precision within an assay): CV%<8% | | | | | Three samples of known concentration were tested twenty times on one plate to assess. | | | Inter-assay Precision (Precision between assays): CV%<10% | | | | | Three samples of known concentration were tested in twenty assays to assess. | | | | | | | | | |
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| Linearity | | To assess the linearity of the assay, samples were spiked with high concentrations of human MR-proANP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | | Sample | Serum(n=4) | | | 1:1 | Average % | 94 | | | Range % | 85-97 | | | 1:2 | Average % | 100 | | | Range % | 94-105 | | | 1:4 | Average % | 90 | | | Range % | 83-94 | | | 1:8 | Average % | 97 | | | Range % | 91-101 | |
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| Recovery | | The recovery of human MR-proANP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
| Sample Type | Average % Recovery | Range | | | Serum (n=5) | 95 | 89-98 | | | EDTA plasma (n=4) | 102 | 92-107 | | | | | | | | | | | | | | | |
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| Typical Data | | These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
 | pmol/L | OD1 | OD2 | Average | Corrected | | | 2500 | 2.155 | 1.988 | 2.072 | 1.926 | | | 1250 | 1.697 | 1.627 | 1.662 | 1.516 | | | 625 | 1.160 | 1.088 | 1.124 | 0.978 | | | 312 | 0.692 | 0.708 | 0.700 | 0.554 | | | 156 | 0.414 | 0.425 | 0.420 | 0.274 | | | 78 | 0.283 | 0.277 | 0.280 | 0.134 | | | 39 | 0.197 | 0.191 | 0.194 | 0.048 | | | 0 | 0.148 | 0.144 | 0.146 | | |
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| Troubleshooting and FAQs | ELISA kit FAQs |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Lead Time | 3-5 working days |