Target Name | interferon alpha-2(IFNA2) |
Alternative Names | Ifna2Interferon alpha-2 ELISA Kit; IFN-alpha-2 ELISA Kit |
Abbreviation | IFNA2 |
Uniprot No. | P01573 |
Species | Mus musculus (Mouse) |
Sample Types | serum, plasma, tissue homogenates, cell lysates |
Detection Range | 9.38 pg/mL-600 pg/mL |
Sensitivity | 2.35 pg/mL |
Assay Time | 1-5h |
Sample Volume | 50-100ul |
Detection Wavelength | 450 nm |
Research Area | Immunology |
Assay Principle | quantitative |
Measurement | Sandwich |
Precision | Intra-assay Precision (Precision within an assay):CV%<8% | | | | | Threesamples of known concentration were tested twenty times on one plate toassess. | | | Inter-assay Precision (Precision between assays): CV%<10% | | | | | Threesamples of known concentration were tested in twenty assays to assess. | | | | | | | | | | | |
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Linearity | Toassess the linearity of the assay, samples were spiked with highconcentrations of mouse IFNA2 in various matrices and diluted with the SampleDiluent to produce samples with values within the dynamic range of the assay. | | | Sample | Serum(n=4) | | | 1:1 | Average % | 90 | | | Range % | 85-94 | | | 1:2 | Average % | 102 | | | Range % | 98-107 | | | 1:4 | Average % | 84 | | | Range % | 80-88 | | | 1:8 | Average % | 95 | | | Range % | 95-99 | | |
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Recovery | Therecovery of mouse IFNA2 spiked to levels throughout the range of the assay invarious matrices was evaluated. Samples were diluted prior to assay asdirected in the Sample Preparation section. | | | Sample Type | Average % Recovery | Range | | | Serum (n=5) | 93 | 89-98 | | | EDTA plasma (n=4) | 98 | 94-102 | | | | | | | | | | | | | | | | | | |
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Typical Data | Thesestandard curves are provided for demonstration only. A standard curve shouldbe generated for each set of samples assayed. | | |  pg/ml | OD1 | OD2 | Average | Corrected | | | 600 | 2.651 | 2.536 | 2.594 | 2.440 | | | 300 | 2.049 | 1.928 | 1.989 | 1.835 | | | 150 | 1.376 | 1.315 | 1.346 | 1.192 | | | 75 | 0.892 | 0.952 | 0.922 | 0.768 | | | 37.5 | 0.568 | 0.529 | 0.549 | 0.395 | | | 18.75 | 0.374 | 0.364 | 0.369 | 0.215 | | | 9.4 | 0.237 | 0.241 | 0.239 | 0.085 | | | 0 | 0.150 | 0.157 | 0.154 | | | |
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Troubleshooting and FAQs | ELISA kit FAQs |
Storage | Store at 2-8°C. Please refer to protocol. |
Lead Time | 3-5 working days |