Target Name | Complement 4(C4) |
Abbreviation | C4 |
Species | Bos taurus (Bovine) |
Sample Types | serum, plasma, tissue homogenates |
Detection Range | 0.156 μg/mL-10 μg/mL |
Sensitivity | 0.078 μg/mL |
Assay Time | 1-5h |
Sample Volume | 50-100ul |
Detection Wavelength | 450 nm |
Research Area | Others |
Assay Principle | quantitative |
Measurement | Competitive |
Precision | Intra-assay Precision (Precision within an assay):CV%<8% | | | | | Threesamples of known concentration were tested twenty times on one plate toassess. | | | Inter-assay Precision (Precision between assays): CV%<10% | | | | | Threesamples of known concentration were tested in twenty assays to assess. | | | | | | | | | | | |
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Linearity | Toassess the linearity of the assay, samples were spiked with highconcentrations of bovine complement 4 in various matrices and diluted withthe Sample Diluent to produce samples with values within the dynamic range ofthe assay. | | | Sample | Serum(n=4) | | | 1:100 | Average % | 95 | | | Range % | 85-101 | | | 1:200 | Average % | 97 | | | Range % | 90-105 | | | 1:400 | Average % | 97 | | | Range % | 86-110 | | | 1:800 | Average % | 95 | | | Range % | 86-99 | | |
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Recovery | Therecovery of bovine complement 4 spiked to levels throughout the range of theassay in various matrices was evaluated. Samples were diluted prior to assayas directed in the Sample Preparation section. | | | Sample Type | Average % Recovery | Range | | | Serum (n=5) | 93 | 89-100 | | | EDTA plasma (n=4) | 96 | 87-102 | | | | | | | | | | | | | | | | | | |
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Typical Data | Thesestandard curves are provided for demonstration only. A standard curve shouldbe generated for each set of samples assayed. | | |  μg/ml | OD1 | OD2 | Average | | | | 10 | 0.245 | 0.249 | 0.247 | | | | 5 | 0.386 | 0.402 | 0.394 | | | | 2.5 | 0.538 | 0.552 | 0.545 | | | | 1.25 | 0.805 | 0.801 | 0.803 | | | | 0.625 | 0.985 | 0.997 | 0.991 | | | | 0.312 | 1.248 | 1.262 | 1.255 | | | | 0.156 | 1.577 | 1.593 | 1.585 | | | | 0 | 2.399 | 2.428 | 2.414 | | | |
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Troubleshooting and FAQs | ELISA kit FAQs |
Storage | Store at 2-8°C. Please refer to protocol. |
Lead Time | 3-5 working days |