SARS-CoV-2 N Antibody Pair 2

Datasheet

Code	CSB-EAP33255A2
Size	US$1000
Image	CSB-EAP33255A2 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). An antibody specific for SARS-CoV-2 Nucleoprotein (N) has been pre-coated onto the microwells. The SARS-CoV-2 Nucleoprotein (N) protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody HRP conjugated specific for SARS-CoV-2 Nucleoprotein (N) is added to detect the captured SARS-CoV-2 Nucleoprotein (N) protein. Followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.
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Target Data

Product Details

Uniprot No.	P0DTC9
Alternative Names	Nucleocapsid protein, NC, protein N, N
Species Reactivity	Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)
Immunogen	Recombinant Human Novel Coronavirus Nucleoprotein (N) (1-419aa)
Immunogen Species	Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)
Concentration	It differs from different batches. Please contact us to confirm it.
Form	Liquid
Protein Names	Human Novel Coronavirus Nucleoprotein (N)
Host	Capture: MouseDetection: Mouse
Buffer	Capture: 50% Glycerol, 0.01M PBS, PH 7.4Detection: 50% Glycerol, 0.01M PBS, PH 7.4
Components	Capture: CSB-EAP33255A2CDetection: CSB-EAP33255A2B(HRP)Reagents are sufficient for at least 5 x 96 well plates using recommended protocol.
Tested Applications	S-ELISA
Troubleshooting and FAQs	Antibody FAQs
Notes	We recommend using the capture antibody at a concentration of 1ug/ml and the detection antibody at a concentration of 0.42ug/ml. Optimal dilutions should be determined experimentally by the researcher.
Lead Time	Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Data

Function	Nucleoprotein packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. It plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Coronavirus nucleoproteins are phosphoproteins, and are encoded near the 3′ end of the genome. N possesses two RNA-binding domains: an N-terminal domain with adjacent S/R-rich motif and the C-terminal 209 amino acids. N protein is invovled in coronavirus infection with many ways: the C-terminal domain (CTD) of N is important for binding the genomic RNA packaging signal leading to selective genome incorporation, the N3 domain interacts with the endodomain of M to form virions, and the serine–arginine repeat region of N (SR) interacts with the first ubiquitin-like domain of nsp3 in a critical early replication step. Moreover, it has also been demonstrated that N can oligomerize through interactions in the CTD, bind viral RNA through the N-terminal domain, unwind double-stranded nucleic acid in the manner of an RNA chaperone, and pack in a helix through the N-terminal domain, though none of these other functions has yet been demonstrated to be important for infection.
Subcellular Location	Virion i UniRule annotation, Host endoplasmic reticulum-Golgi intermediate compartment i UniRule annotation, Host Golgi apparatus
Protein Families	Betacoronavirus nucleocapsid protein family

品牌介绍

武汉华美生物工程有限公司成立于2007年12月，注册资金为5000万元，是一家集科研、生产、销售为一体的国家高新技术企业，以食品安全检测产品生产、诊断试剂原料供应、科研试剂生产、生物技术服务为核心，主要为国内外知名的医药研发公司、诊断试剂生产厂家、政府监管检测机构、高等院校、企业及科研院所等提供相关产品及定制化的技术服务。公司拥有6个研发平台，分别为食品安全检测产品开发平台、高通量基因克隆平台、蛋白质组学的生物标记物高通量筛选平台、生物标记物的免疫性检测方法快速建立平台、四大表达系统的重组蛋白表达平台和科研及诊断用单多抗制备平台。以技术为核心、视质量为生命、奉用户为上帝，竭诚为您提供性价比较高的检测产品及无微不至的售后服务。业务范围主营基因、重组蛋白、抗体、ELISA试剂盒、食品安全检测试剂盒及诊断试剂原料产品。

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